Western blotting is easy with thermo scientific products pdf. Not recommended or intended for diagnosis of disease in humans. Optimized for use with pvdf and nitrocellulose membranes. Pierce ecl plus substrate is a chemiluminescent and chemifluorescent substrate to detect peroxidase activity from hrpconjugated antibodies and probes by western blotting using xray film, ccd camera documentation systems or laserbased imagers. For example, our supersignal west pico plus substrate is recommended. The thermo scientific pierce fast western blot kit, ecl substrate contains optimized reagents that shorten the time to perform a typical western blot from 4 hours to 55 minutes. Plus you wont need to worry about reoptimizing your blots with a new substrate. No single substrate performs best for all western blot systems and meets all sensitivity requirements. Instructions pierce fast western blot kit, ecl substrate.
Sustained luminescence signal is sustained for over. Lowpicogram detection with supersignal west pico plus. While working at purdue university, one of the kits i used for detection of horseradish peroxidasewestern blots was pierces ecl substrate. For detection with ecl plus when using hybond ecl membrane we have found in our laboratories that dilutions of 1.
Ecl plus, dilution factors may need to be greater than previously used with other detection systems. The thermo scientific pierce ecl plus substrate is a chemiluminescent and chemifluorescent peroxidase substrate for western blotting. The term blotting refers to the transfer of biological samples from a gel to a membrane and their subsequent detection on the surface of the membrane. Amersham ecl start western blotting detection reagent amersham ecl start western blotting detection reagent fig 1 is a luminolbased chemiluminescent substrate designed for the detection of proteins immobilized to a membrane. Chemiluminescent substrates for western blotting we offer a broad selection of ecl enhanced chemiluminescence substrates for every application.
Pierce ecl plus western blotting substrate fisher scientific. It is a chemiluminescent detection reagent in the amersham ecl product family that is recommended. I had the opportunity to use pierces ecl reagent for viewing proteins which reacted with the primary antibody sc95555, against clara cell protein cc10, from santa cruz biotechnology. Blots can be repeatedly exposed to xray film to obtain optimal results or stripped of the immunodetection reagents and reprobed. Samples were transferred onto pvdf membrane and probed with 0.
Supersignal west femto maximum sensitivity substrate is designed for low femtogramlevel detection by western blot analysis. Western blotting protocol using chemiluminescent substrates. Table of contents page product overview 2 product structure and generation of chemiluminescent signal 2. Thermo scientific pierce ecl western blotting substrate is a valuepriced, entrylevel peroxidase substrate for enhanced chemiluminescence ecl that directly replaces costlier products without the need to reoptimize conditions. Pi46428 restore plus western blot stripping buffer 30 ml suf. Ecl western blotting substrate is compatible with pvdf and nitrocellulose membranes and various blotting buffers.
In order to obtain the best result, it is necessary to optimize the concentration of primary and second antibodies. Because the luminol and peroxide reagentformulations are identical to other commercially available substrate. It uses an enhanced chemiluminescent substrate for western blotting that was developed for film imaging and is also compatible with ccd imaging. Clarity western ecl substrate, biorad pierce ecl, thermo scientific. Thermo scientific pierce ecl western blotting substrate is a valuepriced, entrylevel horseradish peroxidase hrp substrate for enhanced chemiluminescence ecl that directly replaces costlier products without the need to reoptimize conditions. Pierce ecl substrate 42 supersignal chemiluminescent 4347 substrates and kits lumiphos chemiluminescent substrate 48 quick reference substrate guide 48 data imaging 49 thermo scienti. Pierce ecl plus western blotting substrate characteri. Westernbright ecl is a horseradish peroxidase substrate optimized for chemiluminescent western blots imaged using xray film. Supersignal west femto maximum sensitivity substrate. Thermo scientific pierce ecl 2 western blotting substrate. Enhanced chemiluminescence ecl is a highly sensitive method for detecting proteins bound to western blotting membranes. Thermo scientific pierce ecl plus substrate is an acridanbased chemiluminescent and chemifluorescent hrp substrate for western blot detection using xray film or ccd or laserbased imagers. Pierce ecl plus substrate generates acridinium esters when it reacts with hrp. Ecl western blotting substrate is a highly sensitive, nonradioactive, enhanced luminolbased chemiluminescent substrate for easy detection of horseradish peroxidase hrp on immunoblots.
Chemiluminescent western blotting technical guide and protocols or our supersignal substrate western blotting handbook for a detailed western blotting protocol. High sensitivity ecl substrate kit ab3406 is designed for the detection of proteins with 23pg187ng of protein per band. Ecl western blotting substrate infinity ecl detection reagents are nonradioactive, luminolbased chemiluminescent substrates, developed for the detection of immobilized proteins and nucleic acids conjugated with horseradishperoxidase hrp. Blots can be repeatedly exposed to xray film to obtain optimal results or stripped of the.
This product is sold as pierce ecl 2 substrate cat. This substrate should not be in the light for a long time, please protect it from light, and manipulate in dark room. Pierce ecl 2 substrate is a chemiluminescent and chemifluorescent substrate to detect peroxidase activity from hrpconjugated antibodies and probes by western blotting using xray film, ccd camera documentation systems or laserbased imagers. Sensitive, long lasting substrate for chemiluminescent western blotting with a strong. Western blotting the pierce way step 4b step 5 step 6 step 7 step 8 3 new. Lumigen ecl plus ps3 is an acridanbased technology chemiluminescent and chemifluorescent substrate for sensitive detection of western blotted proteins that are bound to horseradish peroxidase hrp labeled antibodies highly sensitive approximately 5 pg per band of target protein on western blot. Ecl western blotting detection reagents ge healthcare.
Thermo scientific pierce ecl plus substrate is an acridanbased chemiluminescent and chemifluorescent hrp substrate for western blot detection using xray. Pi46430 restore plus western blot stripping buffer 500 ml suf. Boster western blotting substrate may be used for immunoblots, western blots, dot blots and any blotting application utilizing horseradish peroxidase hrpconjugates. Thermo scientific pierce fast western blot kit, ecl substrate. Use the same blotting conditions when switching from amersham ecl plus western blotting. Ecl western blotting substrate kit k820 biovision, inc. We offer a broad selection of ecl enhanced chemiluminescence substrates for every application. Pi32106 pierce ecl western blotting substrate 500 ml kit. The ecl western blotting substrate detects picogram amounts of antigen, and with the use of photographic or other imaging methods, visualizes the presence. Western blotting handbook and troubleshooting guide.
Thermo scientific pierce ecl 2 western blotting substrate flyer. A comparison between commercial and selfprepared ecl reagents. Clarity and ecl plus provided the best signaltonoise ratios across all protein loads. Usa 800 424 6723 australia 61 2 9914 2800 austria 01 877 89 01 belgium 09 385 55 11 brazil 55 11 5044 5699. Our double enhancer proprietary technology allows for modulation of signal intensity and signal duration. Sep 25, 2007 while working at purdue university, one of the kits i used for detection of horseradish peroxidasewestern blots was pierces ecl substrate. Ecl western blotting detection reagents in combination with hybond ecl membranes form a complete solution for western blot applications.
The ecl western blotting substrate is a highly sensitive nonradioactive, enhanced luminolbased chemiluminescent substrate for the detection of horseradish peroxidase hrp on immunoblots. Ecl western blotting substrate from thermo scientific pierce. Clarity and clarity max ecl western blotting substrates. Each westar substrate is at the top of its respective market segment regarding performanceprice ratio. Pierce ecl plus western blotting substrate thermo fisher.
Detection reagent 1, peroxide solution not available. Thermo scientific pierce ecl 2 substrate is acridanbased, generating. Pierce ecl plus western blotting substrate thermo fisher scientific. Pierce ecl plus western blotting substrate 25 ml pi80197 100 ml pi80196 3 x 100 ml pi80196x3 supersignal west pico plus substrate supersignal west pico plus chemiluminescent substrate 20 ml pi34579 200 ml pi34577 500 ml pi34580 1 l pi34578 description unit size cat. Pierce ecl plus substrate is an acridanbased chemiluminescent and chemifluorescent hrp substrate for western blot detection using xray film or ccd or.
Ecl western blotting detection substrate description note troubleshooting 1. Amersham ecl western blotting detection reagents for 4000 cm2 membrane rpn2106. For convenience and economy, pierce also offers complete western blotting kits thatinclude chemiluminescent substrates, enzymeconjugated antibodies, blocking buffers and standard buffers. Thermo scientific pierce antibodies are developed for. One western blotting luminol reagent package contains 125 ml each solution a and solution b, which combine to form sufficient chemiluminescence reagent for a 2,000 cm. Westar is our product line of ecl hrp substrates for western blotting.
Ecl western blotting substrate offers superior signal and clear background. Immobilon western hrp substrates are a family of three premixed reagents for horseradish peroxidasebased detection that offer significant advantages over other. Hrp chemiluminescent substrates our signals are loud and clear. Single component, readytouse formulations simplify detection for steadfast reproducibility with enhanced. If you are currently using a supersignal chemiluminescent substrate, switching to tanon highsig ecl western blotting substrate requires increasing antigen and antibody concentrations.
Advanstas ecl western blotting reagent is optimized for xray film and digital. Detection of recombinant human crp visualized with visulite ecl western blotting substrate. Hrp chemiluminescent substrates our signals are loud and. Supersignal west pico plus chemiluminescent substrate pub. Ecl plus western blotting reagent pack rpn 2124 before using this product, please read the instructions below for safe handling, storage and disposal. The high sensitivity ecl substrate produces a strong signal with very low.
Chemiluminescent substrates for western blotting sigmaaldrich. Enhanced chemiluminescence ecl for routine immunoblotting. Western blotting luminol reagent is recommended for western blotting enhanced chemiluminescence applications using horseradish peroxidase hrp conjugated secondary antibodies. Thermo scientific pierce fast western blot kit, ecl. Advansta westernbright ecl chemiluminescent hrp substrate. The boster western blotting substrate is a luminolbased chemiluminescent substrate and it can detect horseradish peroxidase hrp at high sensitivity levels low picogram. Western blot results require optimizing the process components and steps. Clarity and clarity max ecl western blotting substrates life. Increasing concentrations of protein adult testis lysate prepared in lysis buffer. Tanon highsig ecl western blotting substrate tanon. Biorads clarity family of ecl substrates provides simple, highperformance solutions for all your western blotting needs. Tanon highsig ecl western blotting substrate enables the detection of picogram amounts of antigen and allows for easy detection of hrp using photographic or other imaging methods. Use the same blotting conditions when switching from amersham ecl substrate or pierce ecl substrate to tanon highsig ecl western blotting substrate. Thermo scientific pierce ecl plus substrate helasds595 1.
Bosters ecl plus western blotting substrate is an ultrasensitive, luminolbased chemiluminescent substrate for the detection of horseradish peroxidase hrp. Thermo scientific pierce fast western blot kit, ecl substrate contains optimized reagents that shorten the time to perform a typical western blot from 4 hours down to approximately 55 minutes. Pierce ecl western blotting substrate provides reliability and performance equivalent to other standard enhanced chemilumescent ecl substrates for detection of horseradish peroxidase hrp enzyme activity, yet costs much less. When combined with optimized antibody concentrations and blocking buffers, supersignal west femto substrate enables detection of target proteins in amounts that are too small to be seen with typical ecl substrates. Amersham ecl western blotting detection reagents for. Tanon highsig ecl western blotting substrate is a highly sensitive nonradioactive, enhanced luminolbased chemiluminescent substrate for the detection of horseradish peroxidase hrp on immunoblots.
S outhern blot is used for transferring d na, n orthern blot for r na and w estern blot for p rotein. Ecl plus supersignal west dura supersignal west pico ecl clarity ecl plus supersignal west dura supersignal west pico signaltonoise ratio ecl 70 60 50 40 30 20 10 0 10. Western blotting is easy with thermo scientific products. Because pierce ecl plus western blotting substrate is the same as amersham ecl plus western blotting detection reagent, there is no need for additional optimization of assay conditions when switching substrates. Chemiluminescent substrates for western blotting sigma. Highly sensitive, nonradioactive, enhanced luminolbased chemiluminescent substrate for easy detection of horseradish peroxidase hrp on immunoblots. Thermo scientific pierce antibodies are developed for a wide variety of application needs. Pierce ecl plus western blotting substrate product number. Thermo scientific pierce ecl plus western blotting substrate. Sensitivity requirements for western blotting may vary depending on the abundance of the target protein, the sample availability, and the quality of the antibodies. If you are currently using a thermo scientific supersignal chemiluminescent substrate, switching to pierce ecl plus. Westernbright ecl is more sensitive than other chemiluminescent substrates and uses up to 10 times less antibody than other substrates, allowing you to. Westernbright ecl is more sensitive than other chemiluminescent substrates and uses up to 10 times less antibody than other substrates, allowing you to save precious antibodies and samples. Lumigen ecl plus ps3 is an acridanbased technology chemiluminescent and chemifluorescent substrate for sensitive detection of western blotted proteins that are bound to horseradish peroxidase hrp labeled antibodies.
The ecl western blotting substrate detects picogram amounts of antigen, and with the use of photographic or other imaging methods, visualizes the presence of hrp. Thermo scientific pierce ecl western blotting substrate. Lumigen ecl plus is invented, developed and manufactured by lumigen. The kit provides all the reagents necessary to complete a western blot being probed with a mouse or rabbit primary antibody. The ecl plus western blotting detection reagents from amersham biosciences provide an improved nonradioactive method for the detection of immobilized.